Extracellular matrix analysis of nifedipine‐induced gingival overgrowth: immunohistochemical distribution
作者:
G. E. Romanos,
C. Schröter‐Kermani,
N. Hinz,
D. Herrmann,
J. R. Strub,
J.P. Bernimoulin,
期刊:
Journal of Periodontal Research
(WILEY Available online 1993)
卷期:
Volume 28,
issue 1
页码: 10-16
ISSN:0022-3484
年代: 1993
DOI:10.1111/j.1600-0765.1993.tb01044.x
出版商: Blackwell Publishing Ltd
关键词: collagen;extracellular matrix;fibronectin;gingival overgrowth;immuno histochemistry;nifedipine
数据来源: WILEY
摘要:
The purpose of this study was to demonstrate the localization of collagen types I, III, IV, V, VI and VII as well as the glycoprotein fibronectin in nifedipine‐induced gingival overgrowth. The slices, after the use of indirect immunofluorescence (incubation with antibodies against these extracellular matrix components), showed a diffuse distribution with the anit‐types I and III in the stroma and fluorescent staining of the basement membranes of the epithelium, blood vessels and nerves with collagen type IV antibodies. The increased number of vessels was localized near the surface of the lesion. Collagen tyep V ‐ seen as a filamentous ‐ and collagen type VI ‐ as microfibrillar ‐ components were also localized in the tissue, showing completely different patterns of distribution. Collagen type V appeared “crater”‐like and type VI displayed a “honeycomb”‐shaped structural model. The blood vessels were not stained but the area around their walls demonstrated an intense fluorescence with these antibodies. Collagen type VII showed a characteristic linear staining near to the epithelial basement membrance. In contrast to this, fibronectin localized with a varied intensity in the different areas of the tissues and presented a “could”‐like structure. This shows differences between the matrix components in nifedipine‐induced hyperplasia and confirms the heterogeneity of the matrix in hea
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