Digital image acquisition in in vivo confocal microscopy
作者:
W. M. Petroll,
H. D. Cavanagh,
M. A. Lemp,
P. M. Andrews,
J. V. Jester,
期刊:
Journal of Microscopy
(WILEY Available online 1992)
卷期:
Volume 165,
issue 1
页码: 61-69
ISSN:0022-2720
年代: 1992
DOI:10.1111/j.1365-2818.1992.tb04305.x
出版商: Blackwell Publishing Ltd
关键词: Confocal microscopy;image acquisition;in vivo;real‐time
数据来源: WILEY
摘要:
SUMMARYA flexible system for the real‐time acquisition ofin vivoimages has been developed. Images are generated using a tandem scanning confocal microscope interfaced to a low‐light‐level camera. The video signal from the camera is digitized and stored using a Gould image processing system with a real‐time digital disk (RTDD). The RTDD can store up to 3200 512 times 512 pixel images at video rates (30 images s−1). Images can be input directly from the camera during the study, or off‐line from a Super VHS video recorder. Once a segment of experimental interest is digitized onto the RTDD, the user can interactively step through the images, average stable sequences, and identify candidates for further processing and analysis. Examples of how this system can be used to study the physiology of various organ systemsin vivoar
点击下载:
PDF
(2398KB)
返 回