Separation and Characterization of Red Blood Cells from Newborns and Infants during the First Trimenon of Life Using a Dextran Density Gradient: Mean Cellular Volume and Mean Cellular Haemoglobin Concentration
作者:
Ludwig Grauel,
Silvia Müller,
Johann Gross,
Ingeborg Syllm-Rapoport,
期刊:
Acta Haematologica
(Karger Available online 1982)
卷期:
Volume 67,
issue 2
页码: 102-108
ISSN:0001-5792
年代: 1982
DOI:10.1159/000207034
出版商: S. Karger AG
关键词: Cell physiology;Density distribution;Erythrocytes;Erythropoiesis;Reticulocytes
数据来源: Karger
摘要:
The adaptation of cell physiological parameters of erythrocytes of the newborn to erythrocytes in later life was studied by cell separation in an isopycnic dextran density gradient. The cell characteristics during the first trimester of life were followed by repeated determinations. The mean cellular haemoglobin concentration of the lightest cells is remarkably less than that of the cells of median density. This is probably due to the ongoing synthesis of haemoglobin in reticulocytes, which are highly concentrated in the top fractions. It was shown that the young cells released from the bone marrow become progressively smaller during this period with a decrease of mean cellular volume (MCV) from 104 μm3 at birth to 85 μm3 at the end of the first trimester. It has been established that this process commences during the first week of life. No difference concerning the alterations of cell parameters, especially MCV, in premature and mature infants was found. It is therefore probable that these alterations are due to changes in the external milieu following birt
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