Spurious isotope effects in enzymatic reactions with tritium labelled substrates
作者:
K. G. Oldham,
期刊:
Journal of Labelled Compounds
(WILEY Available online 1968)
卷期:
Volume 4,
issue 2
页码: 127-133
ISSN:0022-2135
年代: 1968
DOI:10.1002/jlcr.2590040205
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
AbstractTritium labelled substrates are commonly used in enzyme assays on account of their high specific activity, cheapness and availability. Because of certain special effects occurring with tritium labelled compounds, isotope effects may erroneously be inferred from rate measurements using these compounds. These special effects, and recommended methods of detecting and eliminating them, are discussed.
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