Effects of Rat Hepatocytes on Macromolecular Permeability of Bovine Aortic Endothelial Cell Monolayer
作者:
N. Utoguchi,
H. Mizuguchi,
K. Saeki,
K. Ikeda,
S. Nakagawa,
T. Mayumi,
期刊:
International Journal of Microcirculation
(Karger Available online 1996)
卷期:
Volume 16,
issue 2
页码: 105-110
ISSN:0167-6865
年代: 1996
DOI:10.1159/000179157
出版商: S. Karger AG
关键词: Endothelial cell;Permeability;Hepatocyte;Co-culture
数据来源: Karger
摘要:
The aim of this study was to examine whether the hyperpermeable structure of the liver endothelium in vivo is related to the interactions of hepatocytes in a culture system. The permeation of macromolecular FITC-labeled dextran (molecular weight 70,000) through a monolayer of bovine aortic endothelial cells (BAEC), cocultured with rat parenchymal hepatocytes (P-hep), was increased. When the BAEC were cocultured with nonparenchymal hepatocytes (N-hep), the permeability of the BAEC monolayer was not increased. However, when the BAEC were cocultured with a mixture of P-hep and N-hep (PN-hep), the BAEC monolayer was more permeable than when BAEC were cocultured with P-hep alone. The conditioned medium of P-hep did not alter the BAEC monolayer permeability, nor did the extracellular matrix of P-hep alter BAEC permeability. When the BAEC were cocultured with PN-hep, the F-actin content was not altered.These findings suggest that the interaction between hepatocytes and endothelial cells exerts an important effect on the hyperpermeable structure of the liver vessels in vivo.
点击下载:
PDF
(1107KB)
返 回