Ultrastructural Analysis of Major Basement Membrane Types in Rhesus MonkeyMacaca mulattaAcellular Renal Cortex
作者:
Edward C. Carlson,
Kristene K. Surerus,
Dave Hinds,
期刊:
Cells Tissues Organs
(Karger Available online 1986)
卷期:
Volume 125,
issue 1
页码: 14-22
ISSN:1422-6405
年代: 1986
DOI:10.1159/000146130
出版商: S. Karger AG
关键词: Acellular renal cortex;Rhesus monkey;Basement membranes
数据来源: Karger
摘要:
Increasing interest in animal models of human nephropathies have led to a number of renal studies in nonhuman primates. In the current investigation, sequential detergent extraction of cellular elements was carried out on renal cortical tissue blocks from rhesus monkey in an effort to demonstrate clearly the morphological features of major basement membrane (BM) types and their associated extracellular matrix (ECM). LM and TEM views of acellular tissue blocks demonstrate planar arrangements of ECM components, while SEM studies provide striking three-dimensional images of their surface characteristics. All major BM types maintain their in vivo histoarchitectures despite the absence of cells. We propose that the intrinsic structural rigidity of tubular (TBM), Bowman’s capsule (BCBM) and peritubular capillary BM (PTCBM) may be related to their close external association with collagenous fibrils, while glomerular BM (GBM) may be internally supported by a network of mesangial matrix (MM) plates and trabeculae which extend onto internal surfaces of peripheral GBM loops. Thicknesses of rhesus monkey renal BMs show that they are similar to those seen in the laboratory rat and, in general, BCBM > TBM > GBM > PTCBM. We conclude that rhesus monkey renal BMs closely resemble those described by us in the human [J. Ultrastruct. Res. 82: 96–110, 1983] and that this species offers an attractive model for studies of renal diseases of BM origin-notably diabetes melli
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