IMMUNOPEROXIDASE AND ELECTRON MICROSCOPY STUDIES OF STAPHYLOCOCCAL LIPOTEICHOIC ACID
作者:
Per Aasjord,
Arne Grov,
期刊:
Acta Pathologica Microbiologica Scandinavica Section B Microbiology
(WILEY Available online 1980)
卷期:
Volume 88B,
issue 1‐6
页码: 47-52
ISSN:0304-131X
年代: 1980
DOI:10.1111/j.1699-0463.1980.tb02602.x
出版商: Blackwell Publishing Ltd
关键词: Staphylococcal lipoteichoic acid;immunoperoxidase;electron microscopy
数据来源: WILEY
摘要:
Immunoperoxidase technique together with electron microscopy shows that lipoteichoic acid (LTA) ofStaphylococcus aureusCowan I is attached to the membrane and penetrates the whole cell wall. A diffuse zone outside and no peroxidase reaction product inside the wall when whole cells were treated with antibody prior to embedding may indicate (i) that LTA is exposed to reaction with antibodies outside the wall, and (ii) that all or most of the anti‐LTA antibodies used are of the IgM class and thus unable to penetrate the wall. Thin sections of strain Wood 46 showed the same picture as Cowan I, but treatment of whole cells before embedding gave no diffuse zone outside the wall. This may be due to a thicker wall as found by electron microscopy and/or shorter LTA‐chains of strain Wood 46 than those present in the wall of Cowa
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