The mean peripheral artcriovenous difference for dehydroepiandrosterone (DMEA), dehydroepiandroslerone sulfate (DHEA-S), androstenedione (A), Iestosterone (T), 5α dihydrotcstostcronc (DHT), and l7-hydro\yprogcsterone (17- P) has been calculated in 7 nonhirsule and 9 hirsule women. No significant difference was found between peripheral artery and vein concentrations for all steroids measured in both groups of women studied. Since steroid concentrations in peripheral arterial samples arc not expected to differ from those from ovarian and adrenal arteries, it is concluded that peripheral venous steroid levels adequately represent the steroid concentrations of arterial blood delivered to steroid-producing tissue.