The surface ultrastructure and adhesive properties of a fimbriatestreptococcus sanguisstrain and six non‐fimbriate mutants
作者:
D W S Harty,
M D P Willcox,
J E Wyatt,
P C F Oyston,
P S Handley,
期刊:
Biofouling
(Taylor Available online 1990)
卷期:
Volume 2,
issue 1
页码: 75-86
ISSN:0892-7014
年代: 1990
DOI:10.1080/08927019009378131
出版商: Taylor & Francis Group
关键词: Streptococcus sanguis;adhesion;fibrils;fimbriae;ruthenium red staining layer
数据来源: Taylor
摘要:
Streptococcus sanguisFW213 carries peritrichous fimbriae (216±28 nm long) and 6 mutants derived from it lack fimbriae but carry peritrichous fibrils with a mean length of 77–4 + 3–9 nm. Both wild type strain and mutants have a ruthenium red staining layer (≤ 14.5±2.9 nm thick) external to the cell wall at the base of the fibrils and fimbriae. The thickness of this layer is strain dependent. Ruthenium red also stains extracellular masses of material, probably extracellular polysaccharide, but not the fimbriae.S.sanguisstrain FW 213 adheres to saliva‐coated hydroxyapatite and buccal epithelial cells and is not aggregated by saliva. The 6 non‐fimbriate mutants of FW213 adhered poorly to hydroxyapatite coated in heated whole saliva (S‐SHA) but 3/6 mutants adhered to the same extent or higher than the wild type to S‐SHA coated in unheated saliva, indicating that strain FW213 may carry a non‐fimbriate adhesin and that whole saliva contains a heat sensitive adhesin. All the mutants had a significantly thinner ruthenium red staining layer (RRL) external to the cell wall than the wild type strain FW213, while the cell surface hydrophobicity showed that the mutants were all less hydrophobic than the wild type FW213.
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