Correlation between Tumor Proliferation and Tumor Tissue Level of Urokinase-Type Plasminogen Activator
作者:
H. Konno,
T. Tanaka,
Y. Maruo,
N. Nishino,
S. Nakamura,
S. Baba,
A. Takada,
期刊:
European Surgical Research
(Karger Available online 1993)
卷期:
Volume 25,
issue 4
页码: 239-244
ISSN:0014-312X
年代: 1993
DOI:10.1159/000129283
出版商: S. Karger AG
关键词: Urokinase-type plasminogen activator;Carcinogenic embryonic antigen;Tumor proliferation
数据来源: Karger
摘要:
We established a xenotransplanted human colon cancer strain, TK-3, which produces urokinase-type plasminogen activator (U-PA) and carcinoembryonic antigen (CEA) simultaneously. Immunohistochemical staining of U-PA revealed that U-PA was located in the cytoplasm of cancer cells. Using TK-3, we investigated whether the tissue level of U-PA changed when the tumor proliferated locally. The mice were divided into three groups: mice of group A, B and C were sacrificed at 4, 5 and 6 weeks after tumor inoculation, respectively. The tissue level of U-PA was 0.78 ± 0.183 ng/mg protein in group A, 0.95 ± 0.189 in group B and 1.13 ± 0.311 in group C. The values of groups B and C increased significantly compared with those of group A, and the tumor weight in each group showed a similar increase. The level of plasminogen activator inhibitor type 2 also increased (0.14 ± 0.078 ng/mg protein in group A, 0.17 ± 0.096 in group B, 0.24 ± 0.172 in group C). On the other hand, the tissue level of CEA did not change significantly (78 µg/g tissue in group A, 88 in group B, 76 tissue in group C), and no correlation was observed between the tissue levels of U-PA and CEA. These results suggest that U-PA plays an important role not only in metastasis, but also in local tumor proliferation, and that its biological action in the autocrine system is independent
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