SummaryTwelve enzymes and liver proteins were examined by starch and cellulose acetate electrophoresis in two samples of hokiMacruronus novaezelandiaefrom New Zealand. Two polymorphic enzymes, a‐glycerophosphate dehydrogenase and sorbitol dehydrogenase, were found giving a mean heterozygosity of 0.027 over 12 enzyme loci or 0.016 over 15 protein loci. No differences in gene frequency were detected in nine regional samples collected from the New Zealand 200‐mile exclusive economic zone. Together with existing biological information on spawning grounds these data suggest that hoki in the New Zealand fishery form one st