Phaseolus coccineusStorage Proteins II. Electrophoretic Analysis and Erythroagglutinating Activity in Various Cultivars
作者:
M. Durante,
R. Bernardi,
M. C. Lupi,
S. Pini,
期刊:
Plant Breeding
(WILEY Available online 1989)
卷期:
Volume 102,
issue 1
页码: 58-65
ISSN:0179-9541
年代: 1989
DOI:10.1111/j.1439-0523.1989.tb00315.x
出版商: Blackwell Publishing Ltd
关键词: Phaseolus coccineus;seed storage proteins;cultivar identification
数据来源: WILEY
摘要:
AbstractBiochemical analyses of seed storage proteins ofPhaseolus coccineushave been carried out to identify seventeen different cultivars. The electrophoretic patterns in native polyacrylamide gel electrophoresis (PAGE) and denaturing conditions (SDS‐PAGE); evidenced qualitative and quantitative differences for the three major protein components: legumin, vicilin and phytohaemagglutinin (PHA). The results were confirmed by isoelectrofocusing (IEF) analyses. Erythroagglutination tests showed the presence of high agglutinating activity particularly in cultivars with low vicilin content. The experimental results allow one to distinguish all the cultivars by their electrophoretic spectra and agglutinating activitie
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