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Prospects for confocal imaging based on nanosecond fluorescence decay time

 

作者: C. G. Morgan,   A. C. Mitchell,   J. G. Murray,  

 

期刊: Journal of Microscopy  （WILEY Available online 1992）
卷期: Volume 165, issue 1  

页码: 49-60

 

ISSN:0022-2720

 

年代: 1992

 

DOI:10.1111/j.1365-2818.1992.tb04304.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Fluorescence decay time;phase shift/demodulation fluorometry;fluorescence imaging

 

数据来源: WILEY

 

摘要:

SUMMARYA fluorescence microscope has been developed to give contrast on the basis of fluorescence decay time. Two different detection schemes are used, one based on an imaging single photon detector equipped with an external photon correlator and the other using an RF‐modulated intensified CCD camera. Examples of the new contrast are shown, and the possible routes to development of a confocal version of the microscope are discusse

 

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