Set of proteins shows abnormal posttranslational modification in embryos homozygous for dominantT‐mutations
作者:
C.‐H. Thomas Park,
Karen Artzt,
Dorothea Bennett,
期刊:
Developmental Genetics
(WILEY Available online 1989)
卷期:
Volume 10,
issue 1
页码: 53-62
ISSN:0192-253X
年代: 1989
DOI:10.1002/dvg.1020100108
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: MouseT‐locus;Two‐dimensional gel electrophoresis;Embryonic development
数据来源: WILEY
摘要:
AbstractTandTcare dominant mutations in the mouse that affect neuroaxial development when heterozygous and cause embryonic death when homozygous. Embryos were analyzed individually by two‐dimensional gel electrophoresis at 9½ days gestation, 1 day before homozygotes die in utero. A comparison of the protein patterns of mutant homozygotes with those of their littermates revealed a set of proteins (T‐proteins) that showed isoelectric point (pl) polymorphism. All the T‐proteins were more basic in mutant homozygotes. These polymorphisms could be detected, although they were less pronounced, in embryos as young as 7½‐day presomite stages, when it is impossible to distinguish homozygous mutants grossly. Interestingly, the same proteins show a pl shift from basic to acidic in wild‐type embryos during development from 7½ to 9½ days. Thus, it appears that inTandTcmutants a developmentally specific posttranslational acidic modification of these proteins is disturbed. The likely cause of the abnormality is a defect in some mechanism for phosphorylation, since the T‐proteins of wild‐type embryos were shifted to higher pls by phosphatase treatment. This disturbance appears to be localized to axial structures (neural tube, somites, and surrounding mesenchyme) since only these structures, and not the rest of the mutant homozygous embryos, contain abnormally
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