Protein Separation by Cross-Axis Coil Planet Centrifuge with Two Different Types of Coiled Columns
作者:
K. Shinomiya,
Y. Kabasawa,
Y. Ito,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1998)
卷期:
Volume 21,
issue 1-2
页码: 111-120
ISSN:1082-6076
年代: 1998
DOI:10.1080/10826079808001940
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Proteins were separated using a cross-axis coil planet centrifuge equipped with two different types of coiled columns, i.e., toroidal and eccentric coil assemblies. An aqueous-aqueous polymer phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate was used for the separation of cytochrome C, myoglobin and ovalbumin. The toroidal coil assemblies with 1 mm ID tubing yielded the partition efficiency of 170 theoretical plates at the myoglobin peak. A slightly higher efficiency was obtained from the eccentric coil assemblies with the same diameter. The highest partition efficiency of 450 theoretical plates was attained from the eccentric coil assemblies with 0.85 mm ID and 64 mL capacity.
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