AbstractA modified Fite carbon fuchsin stain for Mycobacterium species and other acid-fast bacteria is described. This procedure eliminates uneven stain differentiation and areas of excess stain deposition. Harris hematoxylin is used for the counterstain to allow better nuclear detail. This stain was found to be superior to conventional Fite staining for Mycobacterium species and other acid-fast bacteria that are difficult to visual and for total tissue demonstration. (The J Histotechnol19:133, 1996)