Isolation and Characterization of an Undifferentiated Human Colon Carcinoma Cell Line (MIP-101)
作者:
NilesRichard M.,
WilhelmSally A.,
SteeleGlenn D.,
BurkeBohdana,
ChristensenThomas,
DexterDaniel,
O'BrienMichael J.,
ThomasPeter,
ZamcheckNorman,
期刊:
Cancer Investigation
(Taylor Available online 1987)
卷期:
Volume 5,
issue 6
页码: 545-552
ISSN:0735-7907
年代: 1987
DOI:10.3109/07357908709020314
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractAn undifferentiated human colon carcinoma cell line was established from tumor tissue obtained from metastasis to the liver of colonic adenocarcinoma in a patient with fulminant Dukes D colorectal carcinoma. Histological analysis of the tumor biopsy from the liver confirmed the hospital pathology report of poorly differentiated colonic adenocarcinoma. Explants of this tumor tissue xenografted into a nude mouse were used to establish an epithelioid-like cell culture line, MIP-101. The cell line formed tumors in nude mice that hisologically appeared undifferentiated and did not stain for carcinoembryonic antigen (CEA). No CEA was present either by radio-immunoassay (RIA) of the culture supernatant or by immunoperoxidase staining of the tumors or monolayers. MIP-101 appears to be one of the most undifferentiated human colon carcinoma cells lines available. It should prove useful in the search for markers of undifferentiated colonic cancer and in studies of colonic cancer differentiation.
点击下载:
PDF (2586KB)
返 回