Technique for Human Bone Marrow Harvest
作者:
Richard P. Herrmann,
Richard E. Davis,
期刊:
Acta Haematologica
(Karger Available online 1982)
卷期:
Volume 68,
issue 4
页码: 309-312
ISSN:0001-5792
年代: 1982
DOI:10.1159/000206998
出版商: S. Karger AG
关键词: Bone marrow;Collection;Transplantation
数据来源: Karger
摘要:
The usual technique of harvesting bone marrow for allogeneic or autologous transplantation involves passage of the marrow suspension through discs of stainless steel mesh of increasingly small diameter. We describe a sterile technique which is much less messy and produces a single cell suspension. Potter-Elvehjem homogenizers are used to break up the marrow particles. This procedure has been used successfully in 6 patients where allogeneic transplantation was performed and in 6 harvests of autologous marrow. Marrow cryopreserved in this way contains viable committed stem cells and is not subject to agglutination following subsequent thawing.
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