A precise, sequential staining procedure which demonstrates sulfated and non-sulfated mucopolysaccharides and glycogen in the urodele forelimb is described. The process, a combination of the alcian and PAS techniques, has proven valuable in following histogenic events during forelimb regeneration. Non-sulfated mucopolysaccharides are present in apical cap epithelium, sarcoplasm, and bone matrix. Both alcian blue and PAS responses are apparent in dedifferentiating cartilage. Glycogen was apparent in late blastema cells, mature and to a lesser extent in maturing muscle, and wound epithelium.