Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter
作者:
Claude P. Muller,
Gert Krabichler,
期刊:
Review of Scientific Instruments
(AIP Available online 1988)
卷期:
Volume 59,
issue 9
页码: 1928-1932
ISSN:0034-6748
年代: 1988
DOI:10.1063/1.1140053
出版商: AIP
数据来源: AIP
摘要:
The usefulness and limitations of the Becton–Dickinson fluorescence‐activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.
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