Studies on the Dose Dependence of Endotoxin-Induced in vitro Activation of the Complement System
作者:
O. Roeise,
P. Garred,
T.E. Mollnes,
A.O. Aasen,
期刊:
European Surgical Research
(Karger Available online 1989)
卷期:
Volume 21,
issue 1
页码: 34-41
ISSN:0014-312X
年代: 1989
DOI:10.1159/000129001
出版商: S. Karger AG
关键词: C1 inhibitor;In vitro;Endotoxin;complement system;C3 activation fragments;Terminal complement complex
数据来源: Karger
摘要:
The dose and time dependence of in vitro endotoxin-induced activation of complement was studied in citrated pool plasma at 37 °C. C3 activation fragments (C3act) and the fluid-phase terminal complement complex (TCC) were used as indicators of initial and terminal activation, respectively. At 1 h marked elevation of C3act and TCC were found in plasma tested with the highest doses of endotoxin (2· 109 and 2· 108 ng/l). In test plasmas with 2 · 107 and 2 · 106 ng/l of endotoxin, no sign of activation was seen during the first 4 h, whereas both C3act and TCC values were increased at 12 h. In thest plasma with 2· 105 ng/l of endotoxin and in control plasma no elevation of TCC values were seen during the observation period (24 h), whereas C3act values increased slightly and to the same extent in both at 24 h. Parallel to the increases in C3act and TCC concentrations, the functional C1 inhibitor (C1INH) values decreased. Changes in C1INH values, however, occurred with a time lag of approximately 6 h compared to the increases in C3act and TCC. Our in vitro study showed a dose-dependent endotoxin-induced activation of both the initial and the terminal part of the complement cascade evaluated by C3act an
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