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Stability of adenosine 5'‐triphosphate standards for the luciferin‐luciferase bioluminescence assay

 

作者: OlliH. Tuovinen,  

 

期刊: Journal of Environmental Science and Health . Part A: Environmental Science and Engineering  (Taylor Available online 1978)
卷期: Volume 13, issue 5-6  

页码: 387-390

 

ISSN:0360-1226

 

年代: 1978

 

DOI:10.1080/10934527809374818

 

出版商: Taylor & Francis Group

 

关键词: ATP;luciferin‐luciferase;bioluminescence

 

数据来源: Taylor

 

摘要:

ATP in dilute solutions prepared for use as internal standards in the bioluminescence assay is gradually degraded if not continually kept at 0°C or below. The example in this note indicates over 160% recovery of ATP in samples due to the decreased ATP concentration in the internal standard stock solution over a period of 90 min. The degradation of ATP may be of concern in context of ATP‐analyzers with ATP‐injection systems unless precautions are undertaken to stabilize dilute ATP standards.

 

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