Stability of adenosine 5'‐triphosphate standards for the luciferin‐luciferase bioluminescence assay
作者:
OlliH. Tuovinen,
期刊:
Journal of Environmental Science and Health . Part A: Environmental Science and Engineering
(Taylor Available online 1978)
卷期:
Volume 13,
issue 5-6
页码: 387-390
ISSN:0360-1226
年代: 1978
DOI:10.1080/10934527809374818
出版商: Taylor & Francis Group
关键词: ATP;luciferin‐luciferase;bioluminescence
数据来源: Taylor
摘要:
ATP in dilute solutions prepared for use as internal standards in the bioluminescence assay is gradually degraded if not continually kept at 0°C or below. The example in this note indicates over 160% recovery of ATP in samples due to the decreased ATP concentration in the internal standard stock solution over a period of 90 min. The degradation of ATP may be of concern in context of ATP‐analyzers with ATP‐injection systems unless precautions are undertaken to stabilize dilute ATP standards.
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