Human Soluble Antigens and Their Use in Sperm Antibody Testing*
作者:
SIDNEY SHULMAN,
THOMAS KEANE,
期刊:
American Journal of Reproductive Immunology
(WILEY Available online 1981)
卷期:
Volume 1,
issue 2
页码: 69-72
ISSN:0271-7352
年代: 1981
DOI:10.1111/j.1600-0897.1981.tb00019.x
出版商: Blackwell Publishing Ltd
关键词: Antigen;infertility;agglutination;inhibition of;infertility antigen;sperm antibody
数据来源: WILEY
摘要:
ABSTRACT:Efforts were made to disrupt and solubilize human sperm cells and to evaluate the product for its content of infertility antibody‐related antigens. In the procedure that was developed, a well‐washed sperm sample is treated with 0.1 M dithiothreitol for 60 min, followed by trypsin at 0.1 mg/ml for 30 min, and then by soybean trypsin inhibitor. A mixture of DNAses I and II are added. After centrifuging, the resuspended pellet (RP) and the final supernatant (FS) show several degrees of cellular breakdown. Immunological evaluation was done with a strongly positive human serum containing sperm‐head antibody. From the inhibition of sperm agglutination, we could conclude that the desired soluble antigen was obtained in the FS fra
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