Cell Membrane Polarity of the Epithelial Cells in the Endolymphatic Sac of the Guinea Pig
作者:
P.P.G. van Benthem,
F.W.J. Albers,
J.C.M.J. de Groot,
J.E. Veldman,
E.H. Huizing,
期刊:
ORL
(Karger Available online 1992)
卷期:
Volume 54,
issue 5
页码: 241-245
ISSN:0301-1569
年代: 1992
DOI:10.1159/000276306
出版商: S. Karger AG
关键词: Endolymphatic sac epithelium;Membrane polarity;Glycocalyx cytochemistry
数据来源: Karger
摘要:
The epithelial cells of the endolymphatic sac (ES) were studied in order to characterize their glycocalyx composition. Colloidal thorium and cationized ferritin were used as electron-dense markers to visualize the glycocalyx as well as the basement membrane. In addition, enzymatic digestions were performed to identify the reactive components of the glycocalyx responsible for colloidal thorium labeling. The results indicate that the glycocalyx of the ES epithelial lining is very rich in sialic acid. Furthermore, a marked polarity in glycocalyx reactivity is present between the apical and basolateral membranes, whereas difference in glycocalyx reactivity between the light and the dark cells are not obvious. We speculate that the specific composition and the apparent polarity of the ES epithelial cell glycocalyx are of importance to the equilibrium of electrolytes in the ES lumen.
点击下载:
PDF
(2501KB)
返 回