An isocratic technique was developed for the analysis of luteinizing hormone releasing hormone (LHRH) by high performance liquid chromatography (HPLC) using 210 nm UV detection, ZORBAX ODS column (4.6 mm × 15 cm), mobile phase (86% triethylamine phosphate buffer: 14% acetonitrile), and 1.5 mL/min flow rate. The coefficient of variation (C.V.) for precision and proportionality assays was lower than 5% for all concentrations studied. The detection limit of LHRH was 1 ng/mL.