Xenopus laevisadult females exposed to progressive water deprivation and maintained under dry conditions for two weeks showed a statistically significant increase in serum aldosterone and cortico‐sterone levels in response to osmotic stress. These results are in accordance with those previously obtained by us in males from this typically aquatic species subjected to the same experimental conditions. In females returned to water for one week after a two week period of dehydration, serum aldosterone and corticosterone returned to values approximating those of control.