Use of cRNA probes for the detection of enteroviruses by molecular hybridization
作者:
L. Cova,
H. Kopecka,
M. Aymard,
M. Girard,
期刊:
Journal of Medical Virology
(WILEY Available online 1988)
卷期:
Volume 24,
issue 1
页码: 11-18
ISSN:0146-6615
年代: 1988
DOI:10.1002/jmv.1890240103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: riboprobes;specific viral diagnosis;dot hybridization;enterovirus homology
数据来源: WILEY
摘要:
AbstractSubgenomic fragments of cDNA from poliovirus type 1 were inserted downstream from the SP6 or the T7 promoter in a Gemini riboprobe vector and their in vitro synthesized RNA transcripts were used as radiolabeled probes for the detection of enteroviral RNAs by molecular hybridization. The cRNA transcripts appeared to be more sensitive probes than the corresponding cDNAs. In vitro transcripts of the 5′ noncoding region (5′ nc riboprobe) were able to detect all of 14 reference enterovirus strains tested, as well as human rhinovirus 2, by dot blot hybridization with infected cell lysates. The same riboprobe also detected the enteroviral RNAs present in 16 of 18 samples of successive passages of stools in tissue culture and in some cases even in crude stool extracts. A riboprobe from the VP 1 region detected specifically poliovirus types 1,2, and 3 in lysates of infected cells and in 50% of the infected stool specimens tested. These probes could be of particular interest for the epidemic survey of poliovirus infecti
点击下载:
PDF
(485KB)
返 回