Establishment and characterization of TSGH9201, a human gastric carcinoma cell line that is growth inhibited by epidermal growth factor
作者:
Rong‐Yaun Shyu,
Shun‐Yuan Jiang,
Ming‐Yang Yeh,
Chang‐Chung Wang,
Ming‐Fang Wu,
Horng‐Jyh Harn,
Tzu‐Ming Chang,
期刊:
Journal of Surgical Oncology
(WILEY Available online 1995)
卷期:
Volume 58,
issue 1
页码: 17-24
ISSN:0022-4790
年代: 1995
DOI:10.1002/jso.2930580105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: gastric cancer;epidermal growth factor receptor;transforming growth factor α
数据来源: WILEY
摘要:
AbstractA human signet ring gastric carcinoma cell line TSGH9201 was established in vitro. The cells grew in vitro as a monolayer with polygonal morphology and had a population doubling time of 34 hours. The cells secreted tumor markers CEA and CA 125. They were, however, not tumorigenic in athymic nude mice. Karyotypic analysis demonstrated a near tetraploidy with a modal chromosome number of 98. Northern blotting and immunocytochemical analysis revealed the expression of both transforming growth factor α and high levels of epidermal growth factor receptor. Cell growth was inhibited by the epidermal growth factor in vitro. The cell line may be a useful tool to study autocrine growth regulation through the epidermal growth factor receptor. © 1995 Wiley‐Liss,
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