Failure to Detect Genomic Material of HTLV-I or HTLV-ll in Mononuclear Cells of Italian Patients with Multiple Sclerosis and Chronic Progressive Myelopathy
作者:
E. Merelli,
P. Sola,
R. Marasco,
R. Salati,
G. Torelli,
期刊:
European Neurology
(Karger Available online 1993)
卷期:
Volume 33,
issue 1
页码: 23-26
ISSN:0014-3022
年代: 1993
DOI:10.1159/000116894
出版商: S. Karger AG
关键词: Human T-cell lymphotropic virus type I;Human T-cell lymphotropic virus type II;Multiple sclerosis;Chronic progressive myelopathy;Polymerase chain reaction
数据来源: Karger
摘要:
To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences.
点击下载:
PDF
(1505KB)
返 回