Polymorphonuclear Leukocyte Function during Hemodialysis: Relationship to Complement Activation
作者:
Alfred A. Jacobs, Jr.,
Richard A. Ward,
Samuel R. Wellhausen,
Kenneth R. McLeish,
期刊:
Nephron
(Karger Available online 1989)
卷期:
Volume 52,
issue 2
页码: 119-124
ISSN:1660-8151
年代: 1989
DOI:10.1159/000185613
出版商: S. Karger AG
关键词: Oxidative metabolism;Respiratory burst Hemodialysis;Phagocytosis;CR3;Complement;Flow cytometry
数据来源: Karger
摘要:
Phagocytosis, H2O2 production, and C3bi receptor (CR3) expression by polymorphonuclear leukocytes (PMN) obtained from patients before, during, and after a hemodialysis treatment were evaluated by flow microfluor-ometry. The results were compared to changes in plasma levels of C3adesArg and C5adesArg. Prior to hemodialysis C3adesArg and C5ades Arg levels, CR3 expression and phagocytosis were not different from normal controls. However, both basal and phagocytosis-induced H2O2 production were increased. C3adesArg and C5adesArg were increased after 15 min of dialysis; this was accompanied by transient but significant reductions in PMN count and phagocytosis and increased CR3 expression. No changes in basal or stimulated H2O2 production were observed. We conclude that PMN of hemodialysis patients are primed for an enhanced respiratory burst before dialysis is initiated. Dialysis-induced complement activation after the initiation of dialysis does not further stimulate H2O2 production or enhance the response to phagocytosis. However, complement activation may cause leukopenia and CR3 expression.
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