Characterization of hbeag by physicochemical and immunochemical methods
作者:
David Katz,
Joseph L. Melnick,
F. Blaine Hollinger,
期刊:
Journal of Medical Virology
(WILEY Available online 1980)
卷期:
Volume 5,
issue 1
页码: 87-100
ISSN:0146-6615
年代: 1980
DOI:10.1002/jmv.1890050110
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: HBeAg;purification;affinity to IgG;HBsAg;hepatitis virus
数据来源: WILEY
摘要:
AbstractHBeAg, which is a hepatitis B‐associated antigen, was purified and characterized. The end product from ion exchange, gel filtration and immunoadsorbent columns consisted mainly of immunoglobulin G (IgG) molecules, identified by immunoelectrophoresis and polyacrylamide gel electrophoresis. At least four polypeptides with molecular weights of 55,000, 38,000, 25,000 and 20,000 daltons were detected in four different preparations of HBeAg. Two of these have molecular weights similar to the heavy and light chains of IgG (55,000 and 25,000 daltons). It is postulated that HBeAg may be a small ligand of 20,000 daltons with an affinity for IgG. This affinity, especially if it involves the light chain of IgG, could account for the 38,000 or 42,000 molecular weight polypeptides seen by autoradiograph
点击下载:
PDF
(1140KB)
返 回