Raman microprobe spectroscopy: A new tool for biofouling diagnostics
作者:
A Crossley,
P R Graves,
期刊:
Biofouling
(Taylor Available online 1993)
卷期:
Volume 6,
issue 3
页码: 235-243
ISSN:0892-7014
年代: 1993
DOI:10.1080/08927019309386226
出版商: Taylor & Francis Group
关键词: Raman;biofouling;adsorption;electrochemistry;SERS;biomolecules
数据来源: Taylor
摘要:
Raman vibrational spectra were obtained from crystalline amino acids and acid molecules adsorbed at the surfaces of silver electrodes in aqueous solutions. Results revealed that aromatic acids such as phenylalanine adsorbedviatheir aromatic ring while bases such as alanine were coordinated by their amine functional groups. Sulphur containing acids (cysteine and cystine) were found to bond through their sulphur groups. In all cases, adsorption increased towards the point of zero charge of silver, as would be expected for uncharged species. Similar experiments carried out on α‐amylase solutions showed that the enzyme molecule changes its coordination to the silver surface as a function of electrode potential, indicating that C‐S, C‐N, and aromatic ring functional groups are all present on the outer surface of the enzyme structure.
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