Identification and quantitation of embryonic and three types of fetal hemoglobin produced on induction of the human pluripotent leukemia cell line K‐562 with hemin
作者:
J. E. Fuhr,
E. Bamberger,
C. B. Lozzio,
B. B. Lozzio,
A. E. Felice,
G. Altay,
B. B. Webber,
A. L. Reese,
S. M. Mayson,
T. H. J. Huisman,
期刊:
American Journal of Hematology
(WILEY Available online 1982)
卷期:
Volume 12,
issue 1
页码: 1-12
ISSN:0361-8609
年代: 1982
DOI:10.1002/ajh.2830120102
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: K‐562;embryonic Hb;Hb F;AγT,Gγ,AγIchains;Hb synthesis
数据来源: WILEY
摘要:
AbstractThe hemoglobins synthesized by the pluripotent K‐562 leukemia cell line of human origin after induction with hemin have been isolated by DEAE‐cellulose chromatography and characterized by electrophoresis, high pressure liquid chromatography, and a radio‐immunological assay. Six hemoglobin zones have been observed with the following likely compositions. Zone 1: α2ϵ2, or Hb Gower‐2; zone 2: ζ2ϵ2, or Hb Gower‐1; zone 3: ζ2γ2, or Hb Portland‐I; zone 4: Hb F, or α2γ2; zone 5: a mixture of acetylated Hb Portland‐I and Hb F; zone 6: Hb Bart's, or γ4. The embryonic Hbs (zones 1, 2, and 3) constituted 50%–75% of the total Hb present; the quantities varied from one experiment to the other. Both Hb Gower‐1 and Hb Gower‐2 were present. The γ chain was heterogeneous and contained theGγ,AγI, andAγTtypes in a ratio of about 4:2:1, indicating a heterozygosity for the Ile → Thr substitution at position γ75. The methodology used can be applied for additional studies evaluating quantitative changes in Hb
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