Suppression of Humoral and Cell-Mediated Immune Responses in Vitro by Benzo (A) Pyrene
作者:
UrsoPaul,
GengozianNazareth,
RossiRandall M.,
JohnsonRay A.,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1986)
卷期:
Volume 8,
issue 2
页码: 223-241
ISSN:0892-3973
年代: 1986
DOI:10.3109/08923978609028616
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractThe effect of benzo(a)pyrene (BaP) at different molar (MI concentrations on the in vitro anti-sheep red blood cell (SRBC) plaque (antibody) forming cell (PFCI response and the one-way mixed lymphocyte response (MLR) was tested. Inhibition of the PFC response and the MLR occurred when spleen cells were exposed to a wide range of BaP concentrations from 10–4m to 10–8m. Maximum depression of the responses occurred at 10–5M for PFC production (47% of controls) and for the MLR (19% of controls) as measured by a stimulation index. No significant loss in cell viability was observed at this or lower molar concentrations of BaP. The non-carcinogenic analog of BaP, benzo(e)pyrene, did not suppress PFC responses at comparable concentrations. This in vitro system will facilitate manipulations of T and B lymphocytes and macrophages (adherent cells) in a controlled culture environment for precisely characterizing the sensitivity of these cells and their subpopu-lations on exposure to BaP.
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