Serum samples from 102 veterinarians and 191 pig farmers from the southern part of the Netherlands were investigated for antibodies againstBrucella abortus, Leptospiraspp,Streptococcus suisserotype II, Hantavirus (HV), and lymphocytic choriomeningitis virus (LCMV). All samples were collected in 1993 and stored until this study was performed. The prevalence of antibodies againstB. abortusin veterinarians (4.5%) was significantly higher (P=0.01) than in pig farmers (0%). None of the veterinarians (0%) and only one pig farmer (0.5%) had antibodies againstLeptospira spp.. Furthermore, significantly (P=0.015) more veterinarians (6%) than pig farmers (1%) had antibody titres against muramidase‐released protein (MRP), a protein of pathogenic S. suis serotype II strains. None of the veterinarians and a total of 3 (1.6%) pig farmers had antibody titres against HV. The prevalence of antibodies against LCMV tended to be higher in pig farmers (2.6%) than in veterinarians (0%) (P=0.10). It can be concluded that the prevalence of antibodies against the investigated zoonotic agents in veterinarians and pig farmers is low.