ABSTRACT.The effect of prolonged breast‐feeding on the serum concentrations of vitamin D metabolites, calcium, phosphate, and alkaline phosphatase was studied longitudinally in 7 infants from Northern Norway. They were exclusively breast‐fed for a median of 71/2 months. Three of the mothers were supplemented with vitamin D throughout lactation. All but one of the infants had 25‐hydroxyvitamin D (25‐OHD) levels in the rachitic range (<20 nmol/l) on at least one occasion. Vitamin D supplementation of the mother had no apparent effect on the infants' 25‐OHD levels, but the values increased during summer. The infant who had the lowest 25‐OHD levels also had decreased 1,25‐dihydroxyvitamin D (1,25‐(OH)2D) concentrations, while the others maintained l,25‐(OH)2D levels within normal limits. 24,25‐(OH)2D concentrations were undetectable when the 25‐OHD levels were below 35 nmol/l, but the two metabolites were closely correlated for higher values of 25‐OHD. Low 25‐OHD levels were associated with decreased phosphate concentrations at 6 months. The calcium levels were normal throughout the study period of one year, as were all but two of the alkaline phospatase values. Although none of the infants had clinical or biochemical evidence of rickets, the results suggest that the vitamin D supply from human milk is inadequate, and that routine vitamin D supplementation is advisable for breast‐fed infants who are de