New deposition technique, ‘‘guest‐host anchoring method’’, has been developed for scanning tunneling microscopy (STM) visualization of biological macromolecules with clear discrimination against artifacts. The electroactive protein (cytochrome c3) molecules were embedded in the liquid crystal matrix, and imaged by STM as a function of applied voltage. Those STM images are encouraging for potential application of this new method to the STM imagings of a wide range of uncoated biological macromolecules.