Effect of Azelastine Hydrochloride on Macrophage Chemotaxis and Phagocytosisin Vitro
作者:
Mitsuo Honda,
Kazunori Miura,
Tomio Tanigawa,
期刊:
Allergy
(WILEY Available online 1982)
卷期:
Volume 37,
issue 1
页码: 41-47
ISSN:0105-4538
年代: 1982
DOI:10.1111/j.1398-9995.1982.tb04115.x
出版商: Blackwell Publishing Ltd
关键词: anti‐allergic drug;macrophage chemotaxis;macrophage chemotactic factor;macrophage phagocytosis
数据来源: WILEY
摘要:
Azelastine, a newly synthesized anti‐allergic agent, was tested for its effects on guinea pig macrophage chemotaxis and phagocytosis. As specific macrophage chemo‐attractants, we used macrophage chemotactic factorsaandc; separated and highly purified from inflamed skin sites.Macrophage chemotaxis induced by skin extract or chemotactic factors was significantly suppressed by a low concentration of the agent (1 μg/ml); the effect was dose‐dependent. The inhibition of chemotaxis was reversible, because chemotactic activity was restored when the agents was removed by washing cells before chemotactic assay. Inactivation of chemotactic factors was not detected by mixing azelastine and factorsaandc.Azelastine may directly interact with macrophages to decrease their chemotactic responsiveness.β‐Glucuronidase activity in the medium and macrophages after phagocytosis of polystyrene latex particles was not affected by this agent at concentrations ranging from 1 to 10 μg/ml. The phagocytosis of latex particles or sheep red blood cells opsonized with IgG antibodies (EA) and anchoring of macrophages to substrate were not inhibited and azelastine did not damage the macrophages as determined by lactate dehydrogenase (LDH) rel
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