首页   按字顺浏览 期刊浏览 卷期浏览 Effects of Ethyl Carbamate and its Metabolites on the Antibody Response in Splenocyte C...
Effects of Ethyl Carbamate and its Metabolites on the Antibody Response in Splenocyte Cults from Female Balb/C Mice

 

作者: JeongTae Cheon,   KimHyun Ju,   ChaShin Woo,   ParkJong Il,   ShinHo Chul,   KimDong Hyun,   HanSang Seop,   RohJung Koo,  

 

期刊: Immunopharmacology and Immunotoxicology  (Taylor Available online 1996)
卷期: Volume 18, issue 1  

页码: 91-103

 

ISSN:0892-3973

 

年代: 1996

 

DOI:10.3109/08923979609007112

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

AbstractTo investigate a possible role by cytochrome P450 (P450) in ethyl carbamate-induced immunosuppression, an attempt to assess the ability of ethyl carbamate, its metabolites produced by P450 (i.e., ethyl N-hydroxycarbamate and vinyl carbamate), and methyl carbamate to suppress the polyclonal antibody response induced by bacterial lipopolysaccharide was made in splenocyte cultures isolated from female Balb/C mice. The results showed that vinyl carbamate and ethyl N-hydroxycarbamate were more immunosuppressive compared to ethyl carbamate. A structurally related analogue, methyl carbamate, did not suppress the antibody response. These results indicate that metabolism of ethyl carbamate by P450 may produce more immunosuppressive metabolites as in ethyl carbamate-induced carcinogenicity. A pre-incubation study with phenobarbital-induced liver microsomes in the presence of NADPH-generating system showed that the antibody response was suppressed by ethyl carbamate when splenocytes were pre-incubated with ethyl carbamate and microsomes simultaneously. Moreover, the suppression was completely recovered by the addition of a P450 inhibitor, aminoacetonitrile, in the pre-incubation. Taken together, the present results indicate that metabolism of ethyl carbamate by P450 enzyme(s) may be an important pathway to cause immunosuppression.

 

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