AbstractObjective: The aim of this study was to determine the incidence of HbS and β‐thalassemia in the Northern Jordan.Study Design: The investigation targeted 3 areas of Northern Jordan. Blood samples from 2,290 volunteers were evaluated to determine the prevalence of HbS and β‐thalassemia by preparing a hemolysate for cellulose acetate electrophoresis. An additional 568 newborn samples were collected from the umbilical and analyzed for the presence of HbS using electrophoresis.Results: The overall prevalence of HbS and β‐thalassemia was 4.45% and 5.93%, respectively. The incidence of sickle cell trait in the newborn sample was 3.17%. The prevalence of both HbS and β‐thalassemia was higher in the Al‐Gor area in comparison to Ajloun and Irbid.Conclusion: A better understanding of the demographics of these diseases has the potential to aid in the more efficient utilization of health care resources and improved planning and provision of health