Iodinated Bovine Prolactin
作者:
W.A. Bullough,
M. Wallis,
期刊:
Hormone Research in Paediatrics
(Karger Available online 1977)
卷期:
Volume 8,
issue 1
页码: 37-50
ISSN:1663-2818
年代: 1977
DOI:10.1159/000178778
出版商: S. Karger AG
关键词: Prolactin;Iodination;Mammary gland;Mammary cells;Pigeon crop sac;Receptors;Autoradiography;Immunoassay
数据来源: Karger
摘要:
Iodinated bovine prolactin (2.6 iodine atoms/molecule; labelled with a trace of 125I to give a specific activity of 0.041 µCi/mg) was prepared by the chloramine T method. It was active in two bioassays (pigeon crop sac and dispersed mouse mammary cell), though somewhat less active than the unmodified hormone. In an immunoassay, iodinated prolactin was more effective than the unmodified hormone at displacing 125I-prolactin from antibody. High specific activity 125I-prolactin (1 iodine atom/molecule; 70 µCi/µg) was used for autoradiographic studies on the binding of prolactin to mouse mammary cells. In vivo the labelled hormone found in the mammary gland was associated with membranes of mammary epithelial cells and with alveolar lumen contents. In vitro 125I-prolactin was shown to bind to dispersed mouse mammary epithelial cel
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