InPinus sylvestristhere are two chemotypes—those with (2R,3R)‐trans‐taxifolin 3´‐O‐ß‐D‐glucopyranoside ( =T) and those without this substance. Trees containingTalso contained the biochemically related 3´‐O‐ß‐D‐glucopyranoside of eriodictyol and ampelopsin (EandA,respectively). Analyses ofE, TandAwere performed with HPLC and/or TLC. A rapid extraction with aqueous ethanol followed by TLC was used for identification ofT.The segregation inT‐rich andT‐free trees was determined in twenty‐nine progenies from crosses or selfings inPinus sylvestris.Except for six selfed trees which were assumed to be due to illegitimate fertilization, all data supported a monohybrid inheritance of the presence ofTinP. sylvestrisneedles, withT‐free trees being recessive homozygotes. The concentration ofE, TandAin trees containing these flavonoids is modified by other genes. The analyses of 240 trees forAandEare consistent with a monohybrid inheritance.