A Dot Immunobinding Assay to Detect Anti-α‐gliadin Antibodies in Celiac Disease
作者:
Manuela,
Bittolo Tarcisio,
Not Sandra,
Perticarari Sabina,
Cauci Giorgio,
Graziosi Roberto,
期刊:
Journal of Pediatric Gastroenterology and Nutrition
(OVID Available online 1990)
卷期:
Volume 11,
issue 3
页码: 337-341
ISSN:0277-2116
年代: 1990
出版商: OVID
关键词: Celiac disease;α-Gliadin;IgA and IgG antibodies;Dot immunobinding assay;Nitrocellulose;Enzyme-linked immunosorbent assay
数据来源: OVID
摘要:
A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of α-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples together with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.
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