Pregnancy-Associated Plasma Protein-A-Induced Inhibition of Human Leukocyte Elastase: An Artifact
作者:
Paul Bischof,
Alain Gervaix,
Arielle Meisser,
Suzanne Suter,
期刊:
Gynecologic and Obstetric Investigation
(Karger Available online 1990)
卷期:
Volume 29,
issue 3
页码: 169-172
ISSN:0378-7346
年代: 1990
DOI:10.1159/000293369
出版商: S. Karger AG
关键词: Pregnancy-associated plasma protein A;Leukocyte elastase
数据来源: Karger
摘要:
Pregnancy-associated plasma protein A (PAPP-A), was reported to be an inhibitor in many in vitro systems. Since it was shown that the inhibition of coagulation and complement activity attributed to PAPP-A was in fact due to a contamination by heparin occurring during the purification process, we undertook the present study to see whether the reported PAPP-A-induced inhibition of human leukocyte elastase (HLE) could also be attributed to heparin contamination. PAPP-A was purified from maternal pregnancy EDTA plasma by a method which was previously shown to eliminate contaminating heparin: this preparation was inactive in the HLE assay. But PAPP-A isolated by heparin-Sepharose chromatography, or a PAPP-A-free washing of the heparin-Sepharose column were both inhibitors of HLE. Furthermore the inactive PAPP-A preparation, when incubated with the PAPP-A-free washing of the heparin-Sepharose column, yielded a high molecular weight preparation which inhibited HLE. It is concluded that PAPP-A is not an inhibitor of HLE and that the inhibition of HLE previously attributed to PAPP-A was due to contaminating heparin.
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