A simple and rapid in situ staining method for granulocyte‐macrophage colonies in agar culture
作者:
Der‐Cherng Liang,
Sheau‐Wen Ma,
期刊:
The International Journal of Cell Cloning
(WILEY Available online 1988)
卷期:
Volume 6,
issue 1
页码: 25-29
ISSN:0737-1454
年代: 1988
DOI:10.1002/stem.5530060104
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: CFU‐gm;Granulocyte‐macrophage colony;Colony types;Agar culture;In situ staining;Permanent whole plate
数据来源: WILEY
摘要:
AbstractThis report describes a very simple, quick and effective method for in situ staining of granulocyte‐macrophage colonies in agar cultures and for classifying various colony types. The procedure takes only one minute to fix and a few minutes to stain; a few additional minutes are required for preparation of the permanent whole plate. In this process the Riu stain, a modified Romanowsky stain, is used. Besides the ease and rapidity of this procedure, the identification of colony types appears to be enhanced. Thus, the method seems to be very beneficial in routine observations of colony type
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