摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800731

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800732

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr.E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper. Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness.He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper. Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800733

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

7338 COX THE CHEMICAL EXAMINATION OF FURS IN RELATIOK TO DERMATITIS The Chemical Examination of Furs in Relation to Dermatitis BY H. E. COX M.Sc. YH.D. F.I.C. PART I1 RESULTS OF TESTS ON FURS ALLEGED TO HAVE CAUSED DERMATITIS (Read at the Meeting October 4 1933) SOME time ago I described methods of analysis which had been found useful in the testing of furs suspected of causing dermatitis (ANALYST 1929,54 694). The present paper sets out the result of a rather extended experience of the examination of furs and of experiments which were designed to throw some light on the causes or possible causes underlying the disease. Since 1929 much has been published, both on the chemical and medical aspects; perhaps the more useful contributions have been those of Forster and Soyka ( J .SOC. Dyers and Colowists 1931,47 99); Percival (Lancet 1931 417 and Brit. J . Derm. 1933 49); Mayer and Sulzberger (Arch. fiir Derm. 1931 163 245); Mayer (ibid. 223) and Ingram (Brit. J . Derm., 1932) COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS 739 All those who have experience of the investigation of cases of alleged fur dermatitis will agree that it is often very difficult to ascertain the true cause of the skin trouble suffered by the injured party. There is often doubt whether the irritation was really caused by the fur and if so whether it was caused by a dyestuff and if so by what dye? Further was it due to imperfections in the materials or processes or to the idiosyncrasy of the individual ? Controversy ranges round the question of why certain dyed furs c a w dermatitis; some medical authorities merely say it is due to idiosyncrasy others, that the reaction is allergic.The essence of an allergic reaction seems to be the production in the living cell of specific antibodies by the stimulus of foreign substances; according to Bloch the contact between the invading substance and its specific cellular antibody results in an inflammatory reaction. So far as I am aware no real evidence has ever been adduced of the formation of such substances in relation to dyes or intermediates. It is generally agreed that natural undyed furs do not cause dermatitis, assuming of course that they do not contain other irritant chemicals from dressing or bleaching operations. Percival expresses the opinion quite clearly that the irritant properties are not due to mechanical action.If this be so it would appear from Table I that the clinical diagnosis is not always quite justified; the dermatitis may be certain enough but the prefix “fur” may not be accurate and I venture to think it cannot in general be so without a chemical examination of the suspected fur in order to establish (i) whether the fur was in fact dyed; (ii) if so with what type of dye ; and (iii) whether there is any unoxidised intermediate present ? The reason for this is shown in Table I which gives the results of my examination of 216 furs all alleged to be dyed furs which had caused fur dermatitis. The results do not show what proportion of free base if any was present but merely that the fur had or had not been dyed with a particular substance: TABLE I Para-yhenylenediainine .. Meta-phenylenediamine . . Textile dyes . . . . Vegetable colours . . . . Pyrogallol . . . . . . Para-amino-phenol . . Natural undyed furs Lead salts . . . . . . Para-amino-dime thylaniline Unknown amino compounds . . . . . . . . . . . . . . . . No. 98 27 3 20 3 3 4 9 12 37 216 Percentage 45.4 12.5 1.4 9-3 1.4 1-4 1-8 4-1 3.6 17.1 100.0 It will be noted that the phenylenediamines head the list. The textile dyes are applicable only to certain types of dressing and are not often met with in dermatitis cases. Of the vegetable colours I have included only those which were free from topping with phenylenediamine or other compounds; such topping is fairly common in logwood dyeing and is in my opinion the cause of the dermatitis which has sometimes been attributed t o logwood.There are 12 cases in whic 740 COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS mines were indicated but I could not be sure of their identity.* I particularly wish to call attention to the fact that there were 37 cases of natural undyed furs, in which could be found no trace of irritant material. Is it the fact that some of these furs would cause irritation in the absence of dye or is one to suppose that the disease was caused by some totally different agent? I have sometimes heard it suggested that the outbreak may be due to some obscure reaction from contact with the skin of a particular animal-usually the rabbit-towards which the patient is sensitive.With this possibility in mind it is interesting to observe the types of skin involved in these 216 furs examined. Table I1 shows the kinds of fur involved and their relative frequency: Rabbit Hare Fox Lamb Opossum Moleskin Musquash Seal Skunk Cat Squirrel Marmot Pony Monkey Goat Mink Sable Unknown . . . . * . . . TABLE I1 Dyed 89 5 2 28 11 7 1 5 4 6 4 5 0 0 2 0 1 10 189 -Undyed 4 1 2 2 3 3 0 5 0 6 1 1 1 0 1 0 0 37 F -Total 93 6 4 30 14 10 8 5 9 5 10 6 1 1 2 1 1 10 216 -Although the humble rabbit heads the list there is no reason to think that it is specifically irritant; it is the cheapest and most frequently dyed fur and seldom appears in its natural undyed state.Lamb wool comes next in order of frequency. It is difficult indeed to think of anything specific in wool whichcould cause dermatitis and 28 out of 30 of the furs in these cases were dyed. Lamb dyed with phenylenediamine mixtures appearing as nutria is very popular and lamb blackened with logwood and suitably treated passes as astrakhan. Opossum is very often dyed to represent skunk which probably accounts for its frequent appearance. Musquash is not often dyed and seal is sometimes the subject of a special process involving textile colours. Specimens of lynx and susliki have been included as cat and marmot respectively as they belong to those families and there are 10 of the identity of which I was not certain.It is of interest to notice that pony and goat fur which are rather harsh to the skin do not often appear. There seems no reason to think that the particular type of fur has anything to do with dermatitis if it is not dyed. * The probable reason for this failure to establish the identity of the amino compounds will appear in Part IV COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS 741 In view of the preponderance of the 9-phenylenediamine cases it seems desirable to study this substance in more detail. Out of about 100 cases examined, the amounts of 9-phenylenediamine found in an unoxidised state varied from 0.15 per cent.by weight of the hair down to the merest trace. If it be taken that a black-dyed hair contains about 5 per cent. of oxidised p-phenylenediamine it will be seen that this represents a residue of about 3 per cent. imperfectly oxidised and not washed out. In some cases the amount of free and incompletely oxidised base is so small as to raise doubts whether it could possibly cause dermatitis. The next point is as to the mode of action of p-phenylenediamine. Is it a definite chemical or pharmacological action or must one invoke the mysterious word allergy? Percival in 1931 definitely expressed the opinion that it is an allergic action. I notice that in 1933 he remarks that this theory “presupposes the existence of cell-fixed anti-bodies . . . but as there is no direct evidence of the participation of anti-bodies in the eczema reaction the factor of individual susceptibility or idiosyncrasy so constantly associated with it does not permit of the theory being adopted for all cases.” He shows that on applica-tion of 9-phenylenediamine as a patch test about 4 per cent.of normal individuals react after a latent period varying up to 24 days. Babalien and Reitlinger oppose the theory of allergy and regard the effect as a purely local eczema. One may be permitted to ask if a reaction to a low concentration is allergic is a reaction to a high concentration also allergic or is it pharmacological? It has been observed by Bloch and Steiner Wourlisch (Arch. fiir Derm. 1926 152 283) that when the concentration is increased non-sensitive individuals will react to certain potential irritants; even with primula extracts to which most people are insensitive.100 per cent. of positive reactions were obtained with a sufficiently strong extract applied to 12 persons. It seems to me that we ought not to have recourse to the word allergy until all other possibilities have been considered. It is generally agreed that p-phenylenediamine is a strong poison. The experimental basis for this need not be recited in detail for there is much evidence in the literature. It is a blood poison producing oedema salivation urticaria, eczema and various other unpleasant effects. Sartory and Rousseau (BUZZ. Sci. PharmacoZ. 1912 19 338 520) show that upon intraperitoneal injection into rabbits these various ill-effects and death result whereas the oxidation product, Bandrowski’s base is harmless when so injected.Among many papers dealing with the physiological effects may be mentioned those of Erdmann and Vahlen ( J . SOC. Chem. I d . 1906 68) Erdmann (2. angew. Chem. 1905 1377) Soltmann (J. Pharm. Exp. Therap. 1917 9 391) and 0. S. Gibbs (ibid. 1922 20 221). Opinions differ as to whether the diamine itself or an intermediate oxidation product is the true irritant. I propose to discuss in detail the identity and properties of the oxidation products of p-phenylenediamine in another paper but it is convenient before passing on to the reactions of 9-phenylenediamine with the blood to refer to the detection and determination of traces of the free unoxidised base in furs.Ingram (Zoc. cit.) adheres to the theory of allergy 742 COX THE CHEMICAL EXAMINATION OF FURS IK RELATION TO DERMATITIS The detailed constitution of the final oxidation product of 9-phenylenediamine on fur is not yet fully known but it will be shown in Part IV of this series that, under ordinary conditions there is always included some Bandrowski base on the surface of the fibre. It will be found that on treating furs dyed with diamines with mineral acid some of this base is stripped off and a brown solution is obtained which gives all the reactions of the Bandrowski base and some of those of the original phenylenediamine. Further the colour may be reduced either to a leuco compound or even to the diamine itself by strong reducing agents-a procedure which is often very useful in the identification of the amine.Such decomposition does not occur with water at blood-heat or appreciably with faintly acidified solutions. A t 37" C. in water the brown base is hydrolysed only to the extent of 0.2 per cent. in 24 hours; with 0.1 per cent. acetic acid and normal saline which forms a conveqient artificial sweat mixture having PH approximately 3 the hydrolysis at blood-heat is about 2 per cent. Sheepskin dyed with p-phenylene-diamine and washed free from the base was found to yield 0.2 per cent. of apparent 9-phenylenediamine after 2 hours on the water-bath with N/10 hydrochloric acid. In order to study the degree of decomposition of Bandrowski's base it is necessary to have a means of differentiating it from p-phenylenediamine and determining the latter in the presence of the former.Forster and Soyka describe the indamine and certain other reactions of Bandrowski's base in a manner which would imply that one cannot differentiate the two. I find this is not the case if all traces of 9-phenylenediamine are first removed. The base was carefully prepared by the method given by Erdmann (Ber., 1904 37 2908) and was repeatedly washed with dry ether to remove traces of unoxidised diamine; it had m.pt. 242" C. The base so prepared was crystalline and soluble in cold water to the extent of 0.13 grm. per litre. The pure base gives neither the indamine nor the indophenol reaction and does not form a dichlor-di-imine with hypochlorite. The reactions of a saturated aqueous solution as compared with a corresponding strength of p-phenylenediamine are tabulated below : 9-Phenylene-Keagen ts Bandrowski's diamine added to 1Oc.c.base hydrochloride 1. Indamine [3 drops of aniline chloride (1 per cent.) 2. Indophenol (3 drops of 1 per cent. phenol 3 drops 3. Hydrochloric acid and sodium nitrite 4. Sodium-R salt added to 3 6. One drop of N / 1 hydrochloric acid and 1 C.C. of 6. Ferric chloride and hydrogen sulphide solution and 3 drops femc chloride] sodium hypochlorite) alcoholic solution of p-dimethylaminobenzaldehyde No colour Intense green-No colour Intense violet blue Slight brown with Yellow brown Very slight orange Strong red Dirty brown Deep red No change Lauth's violet acid then bleaches Bandrowski's base is decomposed when boiled with hydrochloric acid and can be reduced by stannous chloride or zinc dust.P-Phenylenediamine in appreciable quantities can be titrated with sodium hypochlorite solution in the manner described by Callan and Henderson ( J . SOG. Chem. Ind. 1919,38,408~) ; the limit of sensitiveness is about 1 C.C. of 0.01 N which is equivalent to 0.18 mgrm. of p-phenylenediamine. In smaller quantities th COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS 743 indamine reaction is most convenient though it is not specific for this diamine. It may be applied quantitatively by direct comparison or with a little less accuracy, by the use of the tintometer if due attention be given to the 9 value of the solution. A solution containing 2 parts per 100,000 gives the following colours when 3 drops of a 1 per cent.solution of aniline hydrochloride and 3 drops of 1 per cent. ferric chloride solution are added to 10 c.c. and the colour is observed in a 1-cm. glass cell in Lovibond’s tintometer: P= * . 1.2 2.0 3.0 4-0 5.0 6.0 7.0 - 5.5 8.5 10.0 10.0 9 - Colour{ ’ * I*2 2 3 3 3 3 The maximum colour is seen at approximately 9 4.5; it is developed within a minute and lasts several minutes before changing. Dichromate is not suitable as the oxidising agent as it gives too green a colour. Working at pH 4.5 the approximate concentrations of 9-phenylenediamine are determinable colorimetri-c a y by reference to a table: p-Phenylenediamine partsper100,OOO 1 2 3 4 5 6 7 8 9 10 Blue . . 6 10 11 12 13 14 15 16 17 18 cofour {Yellow .. . . 2 3 3 3 3 3 4 5 6 8 One cannot expect a high degree of accuracy in such a colorimetric process, as there is some room for variation in judgment of the colours. At concentrations a little above 1 in 10,000 blue flecks of the indamine appear and colorimetry becomes impracticable. Using this colorimetric process it was found that Bandrowski’s base when properly prepared and purified from traces of free p-phenylenediamine gave no indamine reaction when extracted with cold water ; when incubated with natural human sweat at blood-heat for 48 hours it showed only a trace of indamine indicating a hydrolysis of less than 0.1 per cent. The concentration used for this test was 5 mgrms. of base with 0.5 C.C. of sweat. The evidence available seems to show that Bandrowski’s base is harmless, and that it is not decomposed on the fibre by sweat or by moisture such as may result from exposure of the dyed fur to rain.This leads to the corollary that if a fur be once properly washed so that all free p-phenylenediamine is removed, none will subsequently be formed by either of these agencies and in the absence of any other toxic intermediate products there can be no question of dermatitis being caused as a result of anything in the fur. PART 111 THE ACTION OF 9-PHENYLENEDIAMINE ON THE SKIN The question of what physiological reactions may arise if free P-phenylene-diamine is present in a fur is worthy of the closest study because as has already been shown in Part 11 it is by far the most frequent cause of fur dermatitis; and, secondly it may be regarded as the typical member of a group of potentially irritant substances used as fur dyes.The mode of action of P-phenylenediamine if it can be traced is likely to provide the key to the mode of action of the other members of the group including the other substituted diamines and aminophenols 744 COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS Quastel (Biochem. J. 1931 25 620 899) has shown that certain dyestuffs are toxic to bacteria and that certain enzyme actions are inhibited a t very low concentrations of the order of 1 in lo5 or los; it seems reasonable to suppose that such an action must depend upon the absorption of the substance into the cells. Thus there seem to be three aspects to consider in connection with P-phenylene-diamine : (i) The absorption or penetration of the diamine; (ii) the reactions of p-phenylenediamine with the blood or serum; (iii) the presence in the blood or serum of abnormal substances or normal constituents under different conditions.(i) THE PERMEABILITY OF THE SKIN.-If the finger be dipped in a 1 per cent. solution of P-phenylenediamine for a few minutes a dark brown stain is produced which cannot be completely washed off so that evidently the surface layer or epidermis is affected. Dead skin likewise is permeable; experiments with a 1 per cent. solution showed that it diffused out through a sheep’s bladder the skin of which was 0.13 mm. thick in less than a quarter of an hour. The coloured oxidation-products likewise passed through quite rapidly and the skin was dyed black.Fig. 1 Fig. % The same 1 per cent. solution was applied to two white mice; the skin of one was washed with ether t o remove any fat and then painted with the diamine whilst the other was painted without any de-fatting. The first mouse licked itself vigorously and in conse-quence died in a few hours; the second did not lick and suffered no inconvenience. It was killed after 24 hours and the skins of both animals were examined. The epidermis was stained a brownish-black but tests on the inner surface with aniline and ferric chloride showed no trace of 9-phenylenediamine in either case. Transverse sections were cut using the freezing method to avoid any extraction by a solvent ; these were mounted and I endeavoured to develop any diamine absorbed as an indamine which shows as a blue-green colour.Fig. 1 shows the result of such treatment; it is unstained with any dye and it is clearly seen that only the outer This does not appear to be the case with living tissue COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS 745 horny layer is coloured at all. There is no evidence of any penetration even with the de-fatted skin of the white mouse which died. Fig. 2 shows another section of the same skin stained with haematoxylin with the object of observing any change in the tissue or cell structure. It will be seen that there is no abnormal appearance a fact borne out by study under a higher magnification. These observations are in agreement with those recorded by Mayer (Arch. fiir Derm. 1931 163 223) who experimented on a number of guinea-pigs applying a 10 per cent.emulsion of p-phenylenediamine in vaseline to the shaved skin. With non-sensitised animals there was no absorption of colour except at the surface in the horny layer whereas in sensitised animals there was evident infiltration with destruction of epidermal cells though Mayer does not appear to have actually identified the amine or imine in the tissues. I have also experimented on dead skin since it is well known that dead cells are more easily stained than live ones. A mouse was killed (by coal gas) and a piece of its skin removed 24 hours later; this was tied over the end of a smooth glass tube and a few drops of the 1 per cent. solution of 9-phenylenediamine were added to the outer surface.After 24 hours a distinct indamine could be developed on the inner surface and examination of a section showed that the e-phenylene-diamine had penetrated. This confirms the view that whilst living skin does not absorb the diamine dead tissue offers no particular resistance to diffusion. Experiments on the human subject are no doubt desirable to enable definite conclusions to be drawn but the evidence available seems to show that 9-phenylene-diamine does not normally pass into the cells of the living skin; when it does do so, further reactions ensue which may or may not produce dermatitis. REACTIONS WITH THE BLOOD OR SERUM.-e-Phenylenediamine is so reactive a compound that it is hardly likely that its introduction into the blood or cells could fail to cause at least a local disturbance.It is not necessary to suppose a general absorption into the blood stream such as is followed by a general toxaemia e.g. as caused by burns. Its addition to blood causes de-oxygenation, and spectroscopic examination showed the reduction of oxyhaemoglobin to methaemoglobin. Szent-Gyorgyi (Biockem. Zeifsch. 1925 157 79) shows that the purple oxidation compound of 9-phenylenediamine is reduced to a colowless compound by reducing systems in the tissues and that this change can be brought about by living muscle tissue and lactic acid. The first oxidation product quinone-di-imine is reduced back to the amine by substances in cellular tissue. Keilin (Proc. Roy. SOC. 1929 [B] 104 206) shows amongst other things that this amine greatly increases the oxygen-uptake of heart muscle.There can be little doubt that anything which interferes with oxidation-reduction processes or enzyme action in the living cells is deleterious or irritant. Harrison (Biochem. J. 1929 23 982) shows that $-phenylenediamine is oxidised to quinone-di-imine by hydrogen peroxide formed in the living cell and may be reduced again therein by reducing systems in the cell. The poisonous properties of quinone-di-imine are known to be intense; its production may be recognised by the formation of the characteristic coloured compound. The indophenol reaction has been much used for the study of enzyme oxidation-reduction processes ; indophenol is produced from the diamine and phenolic (ii 746 COX T H E CHEMICAL EXAMINATION OF FURS I N RELATION TO DERMATITIS compound only in the presence of a suitable oxygen carrier in accordance with the equation : + 2 0 -3 + 2H,O The reaction can be brought about by weak hypochlorite but not by hydrogen peroxide except in the presence of an oxidase.I have made experiments with human blood blood serum and albumen, and the results show that P-phenylenediamine or quinone-di-imine reacts with blood serum in a manner similar to its reaction with albumen and that the presence of the red corpuscles supplies the oxygen necessary for other reactions such as the production of indamine and indophenols ; the reactions are not instantaneous, but take place in an hour or so at 37" C. Blood serum to which a 1 per cent. solution of 9-phenylenediamine is added develops a deep red colour then the serum sets to a jelly and later a slight precipitate forms.A similar action takes place with white of egg. When blood serum is mixed with saturated aqueous solution of a-naphthol and 1 per cent. 9-phenylenediamine is added there is no formation of indophenol but on adding hydrogen peroxide indophenol results, showing that the serum provides the oxidase. If red blood be used instead of serum indophenol results without the addition of peroxide showing that the haemoglobin supplies the necessary oxygen which with the oxidase from the serum brings about the reaction. Control tests with normal saline showed that the precipitation which ultimately follows is not due to haemolysis. Quinone-di-imine coagulates albumen or blood serum as does P-phenylene-diamine but there is a further interesting point that a dark red jelly is produced which does not proceed to give Bandrowski's base whereas quinone-di-imine added to water quickly produces this base.Some of these reactions are tabulated below: Temperature 37" C. ; 1 per cent. solution of 9-phenylenediamine added to : 1. Blood Serum (u) Normal saline So change slight brown colour (b) Blood serum Deep red colour. Jelly forms and slight precipitate (c) Blood serum+ a-naphthol Slight colour slowly develops (d) Serum+ a-naphthol + H,O Strong indophenol reaction (4 Serum+H.ZO Reddish-black colour then ppt. ; serum jellified 2. Human Blood (a) Blood Haemoglobin reduced to methaemoglobin; sets to ;L jelly and darkens in colour (b) Blood + a-naphthol Slight indophenol reaction (c) Blood + a-naphthol + H202 (d) Blood + tyrosine Intense violet indophenol Strong indophenol reaction; dark brown or black ppt.3. White of Egg (a) Albumen Red colour; jelly forms. (b) Albumen + tyrosine Slight violet colour (c) Albumen + hydrogen peroxide Dark brown-black jelly (a) Albumen + a-naphthol Slight colour onl COX THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS 747 4. Quinone-di-imine added to : (a) Water (b) Blood serum (c) Blood (d) White of egg Brown solution gradually forming black ppt. Dark reddish colour Coagulates and darkens in colour Dark red jelly forms The main point arising from these experiments seems to be that the diamine reacts with the proteins of albumen or serum to form a jelly and an oxidation product; cell oxidases are present and if active oxygen be supplied either by red corpuscles or by peroxide indamines or indophenols are produced when the necessary amine or phenol is also present.In view of the de-oxygenation of the cells and the coagulation of proteins which evidently follows the introduction of 9-phenylenediamine into the tissue it seems remarkable that if there is penetration an irritation is not the rule rather than the exception. (iii) ABNORMAL CONSTITUENTS OF THE SERux-The last point to which 1 wish to draw attention is the possibility of variation in the constituents of the blood or human serum arising from individual health diet and unknown causes. The wide range of reactivity of p-phenylenediamine and its imines suggests how varied are the possibilities.If dermatitis were a necessary consequence of the passage of the irritant base through the skin and its action on normal blood or cellular tissue it would be possible easily to demonstrate it by inducing the disease by injection or applying the amine or imine to the broken skin. I have tried this and found that with an individual who did not react to a 1 per cent. solution applied to the unbroken skin there was no typical dermatitis produced after scarification. The 1 per cent. solution was applied for 2 hours; it caused some irritation and a sore place but the characteristic pustules were not formed. Percival likewise has recorded (Zoc. cit.) that application of his Ursol* by scratch did not generally produce dermatitis-a fact which he cites in support of the theory of allergy.It seems possible even likely that diet affects the substances present in the serum and so has important bearing on the development of dermatitis or its absence. The experiments of Mayer and Sulzberger (Arch. fiir Derm. 1931 163, 246) are most striking in this connection. They worked on about 300 guinea-pigs, using organic arsenicals as well as p-phenylenediamine in rather high concentration, and found that by changing over from summer fodder (with an alkaline ash) to dry winter feed the percentage reaction changed from 12 per cent. to 100 per cent. That this was not due to vitamin-shortage was proved by supplying many of the animals with a vitamin concentrate containing A B C and D. Just to explore one of the many possibilities I have tried the reactions of P-phenylenediamine and quinone-di-imine on tyrosine.This substance as is well known is a widely distributed hydrolytic product of proteins which is not normal in blood but occurs in some pathological states; it is found in muscle whenever there is any decomposition or putrefaction. It seems possible therefore, that it might arise when such a deoxidising agent as 9-phenylenediamine is intro-duced. It is known that tyrosine decomposes in the body into phenol and cresol. * The Ursol A which Percival used was probably acetyl-p-phenylenediamine 748 cox THE CHEMICAL EXAMINATION OF FURS IN RELATION TO DERMATITIS The colour reactions of L-tyrosine with p-phenyleaediamine were found to be as follows when applied to a saturated aqueous solution (2.e.about 1 in 2000) : 1. Tyrosine + p-phenylenediamine 2. Do. do. +ferric chloride Strong green indamine quickly changing to 3. Do. do. + dichromate Green changing to safranin red 4. Do. do. +sodium hypochlorite Intense indophenol violet 5. Do. do. +blood serum Slight violet colour 6 . Do. do. +blood serum+ peroxide Intense indophenol violet quickly formed Slight brown only brown precipitate It will be seen that the reactions observed with blood or with serum and P-phenylenediamine are similar in type to those given when tyrosine is added, though they take place more slowly. It is not suggested that this proves anything, but it does illiistrate the possibilities arising in differing individuals or resulting from different diets.In conclusion I think it may be said that the available experimental evidence points to free $-phenylenediamine as the potential irritant which may act through its early oxidation products and condensations. Normally these substances do not pass into the cells or living tissues of the skin but merely stain the horny layer. Dermatitis seems to arise only when the irritant passes into the epidermis and reacts with the serum as a result of special conditions. These conditions include the abnormal permeability of the outer layer and an abnormal chemical or physical constitution of the serum. The coagulation and oxidation effects would preclude its passage into the blood-stream or action at places remote from the site of penetration. SUMMARY.-DetailS are given of the kind of fur and mode of dyeing of 216 furs suspected of having caused dermatitis; of these 37 were undyed and it is thought that they could not have caused the disease. Chemical tests for the differentiation of $-phenylenediamine from Bandrowski’s base are given and a colorimetric method for determining small quantities of the diamine. Experiments are described showing that $-phenylenediamine penetrates the dead skin readily, but does not pass through or into living skin undei normal conditions. Somelof the reactions of p-phenylenediamine with blood and serum are described and it is shown that it coagulates the proteins and becomes oxidised at their expense; the oxidases present in blood suffice to bring about certain reactions and if haemoglobiii is present these reactions proceed further. Tyrosine with albumen produces colour reactions similar to those observed with blood. It is suggested that irritation is determined by the abnormal penetration of the diamine through the skin followed by its local reaction with certain constituents of the blood or serum yet unknown. 11 BILLITER SQUARE LONDON E.C.

ISSN:0003-2654    

DOI:10.1039/AN9335800738

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800749

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800752

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800754

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800756

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800757

出版商:RSC    

年代:1933

数据来源: RSC

摘要:

WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock. Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent. Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it.In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given.However, potatoes had been given to horses in limited quantities without bad results. Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes.Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.WILLIMOTT AN INVESTIGATION OF SOLANINE POISONING 439 DISCUSSION The PRESIDENT remarked that it seemed somewhat strange that potatoes in certain states (old potatoes, green potatoes, etc.) were often described as highly toxic to stock.Many hundreds of tons had been used as stock feed, and he had never actually known of any stock poisoning. Of course, it could be argued that the potatoes used for feeding were often cooked, and much of the solanine dissolved out, but, on the other hand, a large number of these potatoes were used uncooked, and, therefore, stock did consume this vegetable with its full content of solanine, and, so far as he knew, without detriment. During the war a circular was issued, stating that potatoes were an injudicious feed for horses, and setting out the maximum amount that should be given. However, potatoes had been given to horses in limited quantities without bad results.Mr. C. E. SAGE said that cases of solanine poisoning were not infrequent, but he knew of no fatal one, caused by eating the bright red berries of Solanum dulcamara, which grew plentifully in this country. Solanum nzgrum, which had white flowers and black fruits, were sometimes eaten by country children, but he had never met with any casualties. Until recently the fruits of Solanum carolinense were used in America for the preparation of a tincture for medicinal use. I t grew plentifully as a weed in the Southern States, and the orange-coloured fruits were known as Sodom apples. The alkaloids, solanine and solanidine, existed in the fruits, and, as the negroes had used it for a long time as a remedy for falling sickness, the alkaloid could not be particularly toxic, although it was undoubtedly potent.Solanum chenopodium was a native of Queensland, and some thirty years ago he had isolated the alkaloid solanine from it. In Brazil, S. aculeatissimum was the plant yielding Sodom apples. It also contained solanine. With all these sources of solanine, and with potatoes and tomatoes containing it, there seemed to be no great danger from its toxicity. The leaves of S. nigrum were boiled and eaten in the Hawaii Islands, and the fruits known as “fox’s grapes’’ could be eaten in small quantities without any harmful effects. With sprouting potatoes it did not seem beyond the bounds of possibility that some product of decomposition might have caused the illness of people recorded in the paper.Mr. W. PARTRIDGE said that his only experience in the determination of solanine was in connection with a case in which it was alleged that potatoes had been tampered with and had caused illness. He had used the Meyer process, as outlined by Harris and Cockburn (ANALYST, 1918,43, 133) , but with the improve- ments (including the correction for solubility in ammonia wash-water) of Borner and Mattis (ANALYST, 1924, 49, 284). The potatoes in question, substantial areas of which were green, contained 19 mgrms. of solanine per 100 grms. Taking the attitude that normal potatoes contained about 2 mgrms., and never niore than 8 mgrms. per 100 grms., and that 25 mgrms. per 100 grms. were known to be dangerous, he had felt justified in associating the symptoms, minor though they were, with green potatoes. Mr. E. M. HAWXINS remarked that there seemed to be something in the wisdom of old-fashioned country folk. Thirty years ago when, fresh from the town, he went to live in a rural district, the old country folk instructed him that a potato which was allowed to get green through exposure above the earth was likely to be dangerous to human beings and to animals, and he, therefore, never used them. He thought it significant, in the Glasgow case, that more solanine was contained in potatoes which were green from exposure to sunlight than in the white tubers, and the paper certainly bore out this same contention.

ISSN:0003-2654    

DOI:10.1039/AN9335800759

出版商:RSC    

年代:1933

数据来源: RSC