摘要:

APseudomonasisolated from soil, capable of growing on a medium containing methanol as the only carbon source, is described. Biotin or one of a few closely related compounds is required in very low concentrations as a growth factor. It is shown that the ability to use methanol is an adaptive system and that the biotin is incorporated in the cell, but is recoverable on acid hydrolysis.

ISSN:0008-4166    

DOI:10.1139/m59-011

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

Of a number of carbohydrates tested, holocellulose from wheat straw andL-arabinose were the better substrates for production of pentosanase byTrichoderma viride.D-Xylose did not induce synthesis of the enzyme by the fungus. Production in a synthetic medium was increased by the addition of malt sprouts, distillers' dried solubles, or glutamic acid.Under the same conditions the production of pentosanase byAspergillus nigerwas favored by the water-soluble pentosan of wheat flour, holocellulose, and wheat bran. BothD-xylose andL-arabinose induced synthesis of enzyme. In the synthetic medium the addition of yeast extract, corn steep liquor, malt sprouts, or a number of other nitrogenous adjuncts increased the yield of pentosanase. A medium containing 3% bran ground to pass a 20-mesh sieve, 3% corn steep liquor neutralized with ammonium hydroxide, and 1%calcium carbonate was developed for the production of pentosanase byA.niger. Maximum yield was obtained in 60 hours. The pentosanase had an optimum pH of 5.0 and was stable for 30 minutes at 30 °C between pH 4.0 and 5.8. The pentosanase could be precipitated from the culture filtrates with 76% ethanol and when stored as a dry powder at 2 °C was stable for at least 1 year.

ISSN:0008-4166    

DOI:10.1139/m59-012

出版商:NRC Research Press    

年代:1959

数据来源: NRC

摘要:

Pseudomonas convexavar.hippuricumcells harvested from hippurate medium were shown to possess the ability to oxidize hippurate, benzoate, salicylate, and catechol without lag phase. Acetone-dried preparations of these cells were found to possess enzymes for oxidizing these substrates. SeveralPseudomonasstrains were demonstrated to have the capacity to use salicylate as a sole source of carbon and energy. In the light of the results obtained on the utilization of benzoate, salicylate, and catechol byP.convexavar.hippuricumstrain grown on those substrates, it is suggested that salicylate is an intermediate in the breakdown of benzoate.

ISSN:0008-4166    

DOI:10.1139/m59-013

出版商:NRC Research Press    

年代:1959

数据来源: NRC

ISSN:0008-4166    

DOI:10.1139/m59-014

出版商:NRC Research Press    

年代:1959

数据来源: NRC

ISSN:0008-4166    

DOI:10.1139/m59-015

出版商:NRC Research Press    

年代:1959

数据来源: NRC