摘要:

Washings of mixtures of maggots ofCalliphora erythrocephala,C.latifrons,C.vomitoria nigribardaandCynomya cadaverina, and ofC.erythrocephalaalone, showed bactericidal activity againstStaph.aureus,Strep.mastitidis, two strains ofBrucella abortus, andB.typhosus in vitro. Flies were successfully raised in the laboratory but washings from their maggots became less active with each generation. The pH value of active samples was generally greater than that of poor samples. Attempts to increase activity by rendering poor washings alkaline were unsuccessful. Diluted washings showed some bactericidal power. Keeping quality of liquid samples was poorer than that of dried ones. Washings from meat in which maggots had been grown had a definite bactericidal value, while those from control samples of meat had none. A suspension of maggots ground after washing was also inactive. Addition ofBr.abortusto meat on which maggots were grown did not increase bactericidal activity against this organism. Filtration reduced bactericidal action and toxicity. Intraperitoneal injections were toxic to guinea pigs. An anti-maggot rabbit serum had good complement fixing power with an antigen of maggot washings. Intraperitoneal injections of diluted washings failed to protect guinea pigs against infection withBr.abortus, but there was some delay in development of infection, as indicated by slower appearance of agglutinins in treated animals.

ISSN:1923-4287    

DOI:10.1139/cjr38d-021

出版商:NRC Research Press    

年代:1938

数据来源: NRC

摘要:

Guinea pigs were protected against infection withBrucella abortusby intraperitoneal injections of fresh, unpreserved, anti-abortus rabbit serum. In the first two experiments the serum-treated and control animals were exposed to infection by contact with infected guinea pigs, while in the third experiment they were exposed by instillation of the infecting organism into the eye. In the first experiment 60% of the controls and none of the treated animals were infected. In the second, 80% of the controls and 20% of the treated animals were infected. In the third, all of the treated animals were protected, while all of the controls became infected. The former method, although less certain than the latter, is worthy of consideration in experiments of this type, as it permits infection to occur in a natural manner.

ISSN:1923-4287    

DOI:10.1139/cjr38d-022

出版商:NRC Research Press    

年代:1938

数据来源: NRC

摘要:

not available

ISSN:1923-4287    

DOI:10.1139/cjr38d-023

出版商:NRC Research Press    

年代:1938

数据来源: NRC