摘要:

A medium consisting of acid-hydrolysed casein, glycerol, and a salt mixture has been shown to yield pyocyanin byPseudomonas aeruginosaequal in amount to that obtained from glycerol peptone agar. The monoaminomonocarboxylic acids fraction obtained from casamino acids (Bacto) has been shown to be the source of nitrogen essential to pigment formation. Glycine,dl-alanine,dl-valine, orl-tyrosine have been shown to produce pyocyanin when employed as sole sources of nitrogen. The addition ofl-leucine to media containing glycine ordl-alanine markedly increased pyocyanin formation. The addition ofdl-phenylalanine to a synthetic medium inhibited pigment production. A synthetic medium consisting ofdl-alanine or glycine at 0.4% concentration combined with 0.8%l-leucine, 1.0% glycerol, and a salt mixture has been shown to be the most suitable medium for pyocyanin production by five representative strains ofPseudomonas aeruginosa.

ISSN:1923-4287    

DOI:10.1139/cjr47c-012

出版商:NRC Research Press    

年代:1947

数据来源: NRC

摘要:

Potassium either as potassium chloride or dipotassium hydrogen phosphate was found to stimulate production of 2,3-butanediol from starch byBacillus polymyxaand to increase the diol: ethanol ratio. In a casein hydrolysate medium, potassium alone produced this effect; however, in a synthetic (amino acid) medium, phosphorus was found to cause a slight increase in yield of diol especially in the presence of potassium.Potassium, phosphorus, and magnesium were shown to be required for growth ofB.polymyxain a synthetic medium containing glucose, amino acids, and biotin.By means of 'resting cells' ofB.polymyxa, acting on glucose, it was demonstrated that potassium specifically stimulated the diol-synthesizing mechanism and that sodium could replace this element partially.

ISSN:1923-4287    

DOI:10.1139/cjr47c-013

出版商:NRC Research Press    

年代:1947

数据来源: NRC