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THE BIOLOGICAL PURIFICATION OF VACCINE EMULSIONS BY PENICILLIN |
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Canadian Journal of Research,
Volume 24e,
Issue 6,
1946,
Page 149-154
J. Edouard Morin,
Hector Turcotte,
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摘要:
The present communication furnishes a new method for the purification of smallpox vaccine emulsion by penicillin. Penicillin, when added in definite quantities, possesses an undeniable antibiotic power on most of the Gram-positive cocci and on the vegetative forms of certain anaerobic bacteria such asClostridium perfringensandC.fallaxfound in the vaccine. By maintaining a threshold of penicillin (56 units per cc.), the spores of the above mentioned anaerobic microbes cannot grow. When the vaccine emulsion freed from penicillin is inoculated, new strains without any pathogenic properties can be subcultured. Penicillin has no effect on the vaccine virus. By this technique, the vaccine can be freed, in a few days, of its principal pathogenic microbes except from Gram-negative bacilli. In the emulsion thus treated, the periodic control of the penicillin titre is as necessary as the repeated tests made to establish the potency of the vaccine.
ISSN:1923-4287
DOI:10.1139/cjr46e-016
出版商:NRC Research Press
年代:1946
数据来源: NRC
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STUDIES OF SHOCK PRODUCED BY MUSCLE TRAUMA: II. PATHOLOGICAL CHANGES IN VARIOUS TISSUES |
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Canadian Journal of Research,
Volume 24e,
Issue 6,
1946,
Page 155-162
R. A. Cleghorn,
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PDF (788KB)
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摘要:
The following pathological changes were found in the viscera of dogs that died as a result of muscle trauma: (a)Lungs—congestion and patchy consolidation that was not constant or extensive. (b) The mucosa of the uppergastrointestinal tractand descending colon—congestion, haemorrhage, and at times small areas of erosion. (c)Liver—congestion of the sinusoids in dogs that died soon after trauma; in those dying later than three hours after trauma, degeneration of cord cells in the region of the central vein. Animals surviving longer than 18 hr. showed little or no cellular degeneration. (d)Kidneys—congestion of capillary tufts and other small vessels was seen in those dogs dying soonest. Sections from dogs that died three hours or more after trauma showed various degrees of degenerative changes in the tubules. (e)Adrenal cortex—congestion was apparent in animals dying within an hour of trauma. In later deaths, infiltration of leucocytes and degeneration of cells were notable features. Gross haemorrhages were present in dogs that died after five hours. Severe cortical changes were found in two dogs that bled to death accidentally at 8 and. after trauma despite a well maintained blood pressure up to the time of death; mild renal degenerative changes were present in both; a liver section obtained from one of these dogs appeared practically normal. (f)Pancreas—little congestion was seen in dogs that died within five hours after trauma but was more evident in dogs that died later and, in this respect, paralleled the gross changes in the adrenal glands.The findings are discussed and it is concluded that absorption of toxic substances from the damaged muscles contributed, along with the secondary effects of oligaemia, to the tissue changes observed.
ISSN:1923-4287
DOI:10.1139/cjr46e-017
出版商:NRC Research Press
年代:1946
数据来源: NRC
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